
We found that glutamine usage is repressed by rapamycin remedy Figure 1 and SIRT4 is brought on by mTORC1 inhibition Figure 2. Thus, we validated whether SIRT4 itself directly regulates mobile glutamine uptake. The reliable expression of SIRT4 ended in matlab repression of glutamine uptake in Tsc2−/− MEFs and DLD1 cells Figures 5A and 5B. Glucose uptake was not plagued by SIRT4 expression data not proven. Because glutamine can also be a vital nutrient for energy creation, we tested ATP levels in SIRT4 expressing cells. Consistent with decreased glutamine intake, matlab expression of SIRT4 in Tsc2−/− cells led to lowered ATP/ADP ratio in comparison to handle cells Figure 5C. 794 77 . 257 Total 19. 879 79 ANNEXURE VI: INDEPENDENT SAMPLES TEST t test for Equality of Means Levene’s Test for Equality of Variances F Sig. t df Sig. 2 tailed . 046 .